[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological characteristics].
Identifieur interne : 004F02 ( Main/Exploration ); précédent : 004F01; suivant : 004F03[The cloning, expression, purification and identification of SARS virus S2 gene and study on its immunological characteristics].
Auteurs : Xue-Yuan Dong [République populaire de Chine] ; Xiao-Ang Yang ; Yan Li ; Guo-Bin Xu ; Zhen-Ru Feng ; Yue-Dan Wang ; Wei-Feng ChenSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2004.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (analyse), Clonage moléculaire, Escherichia coli (génétique), Escherichia coli (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (isolement et purification), Humains, Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (isolement et purification), Protéines recombinantes (biosynthèse), Protéines recombinantes (isolement et purification), Protéines virales (biosynthèse), Protéines virales (immunologie), Protéines virales (isolement et purification), Régulation de l'expression des gènes viraux, Sous-unités de protéines (biosynthèse), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (sang), Sérum (métabolisme), Thiorédoxines (biosynthèse), Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- analyse : Anticorps antiviraux.
- biosynthèse : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Protéines virales, Sous-unités de protéines, Thiorédoxines.
- génétique : Escherichia coli, Virus du SRAS.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines virales, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Protéines virales.
- métabolisme : Escherichia coli, Sérum.
- sang : Syndrome respiratoire aigu sévère.
- Clonage moléculaire, Glycoprotéine de spicule des coronavirus, Humains, Régulation de l'expression des gènes viraux, Virus du SRAS.
English descriptors
- KwdEn :
- Antibodies, Viral (analysis), Cloning, Molecular, Escherichia coli (genetics), Escherichia coli (metabolism), Gene Expression Regulation, Viral, Humans, Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (immunology), Membrane Glycoproteins (isolation & purification), Protein Subunits (biosynthesis), Recombinant Proteins (biosynthesis), Recombinant Proteins (isolation & purification), SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (immunology), Serum (metabolism), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Thioredoxins (biosynthesis), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (immunology), Viral Envelope Proteins (isolation & purification), Viral Proteins (biosynthesis), Viral Proteins (immunology), Viral Proteins (isolation & purification).
- MESH :
- chemical , analysis : Antibodies, Viral.
- chemical , biosynthesis : Membrane Glycoproteins, Protein Subunits, Recombinant Proteins, Thioredoxins, Viral Envelope Proteins, Viral Proteins.
- blood : Severe Acute Respiratory Syndrome.
- chemistry : SARS Virus.
- genetics : Escherichia coli, SARS Virus.
- chemical , immunology : Membrane Glycoproteins, SARS Virus, Severe Acute Respiratory Syndrome, Viral Envelope Proteins, Viral Proteins.
- chemical , isolation & purification : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins, Viral Proteins.
- metabolism : Escherichia coli, Serum.
- Cloning, Molecular, Gene Expression Regulation, Viral, Humans, Spike Glycoprotein, Coronavirus.
Abstract
To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.
PubMed: 15193211
Affiliations:
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Le document en format XML
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first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name></author><author><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name></author><author><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15193211</idno><idno type="pmid">15193211</idno><idno type="wicri:Area/PubMed/Corpus">002D43</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" 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first="Xue-Yuan" last="Dong">Xue-Yuan Dong</name><affiliation wicri:level="4"><nlm:affiliation>Department of Immunology, Health Science Center, Peking University, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Health Science Center, Peking University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Yang, Xiao Ang" sort="Yang, Xiao Ang" uniqKey="Yang X" first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name></author><author><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name></author><author><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (analysis)</term><term>Cloning, Molecular</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Gene Expression Regulation, Viral</term><term>Humans</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (isolation & purification)</term><term>Protein Subunits (biosynthesis)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (isolation & purification)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Serum (metabolism)</term><term>Severe Acute Respiratory Syndrome (blood)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Thioredoxins (biosynthesis)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (isolation & purification)</term><term>Viral Proteins (biosynthesis)</term><term>Viral Proteins (immunology)</term><term>Viral Proteins (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (analyse)</term><term>Clonage moléculaire</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (isolement et purification)</term><term>Humains</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (isolement et purification)</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines virales (biosynthèse)</term><term>Protéines virales (immunologie)</term><term>Protéines virales (isolement et purification)</term><term>Régulation de l'expression des gènes viraux</term><term>Sous-unités de protéines (biosynthèse)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (sang)</term><term>Sérum (métabolisme)</term><term>Thiorédoxines (biosynthèse)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Membrane Glycoproteins</term><term>Protein Subunits</term><term>Recombinant Proteins</term><term>Thioredoxins</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Protéines virales</term><term>Sous-unités de protéines</term><term>Thiorédoxines</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines virales</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Proteins</term><term>Viral Envelope Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Serum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Sérum</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Humans</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Régulation de l'expression des gènes viraux</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To express S2 protein of SARS virus fused with Trx and then detect its reactivity to the sera from convalescent SARS patients.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><noCountry><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name><name sortKey="Yang, Xiao Ang" sort="Yang, Xiao Ang" uniqKey="Yang X" first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Dong, Xue Yuan" sort="Dong, Xue Yuan" uniqKey="Dong X" first="Xue-Yuan" last="Dong">Xue-Yuan Dong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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